SPIE Student Chapter
Seminar Series
hosted by the Institute of Optics
“Second Harmonic Generation Microscopy”
Xiaoxing Han
Graduate Student
Institute of Optics
University of Rochester Medical Center
Tuesday, 11 August 2009
11:00 AM - 12:00 PM
Sloan Auditorium, Room 101, Goergen Hall
University of Rochester
Abstract
Second Harmonic Combination (SHG) is the non-absorptive combination of two excitation photons into one emission photon within a non-centrosymmetric medium such as a crystal surface, or properly ordered fibers. In practice, SHG microscopy is quite similar to Two Photon Excitation Fluorescence (TPEF) microscopy. However, there is acrucial difference between SHG and TPEF. SHG is based onscattering, meaning that it is a coherent phenomenon, whereas TPEF is based on fluorescence (i.e. absorption followed by emission),meaning that it is incoherent. My research work is focused onexploiting these coherent properties of SHG emission to study the molecule structure of collagen fiber in tumor Extra Cellular Matrix (ECM). The two coherent properties I used to study tumor collagen molecule structures are polarization and the ratio of forward vs. backward propagating SHG
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